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brdu cell proliferation kit 2750  (Millipore)


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    Millipore brdu cell proliferation kit 2750
    Silencing of C/EBPβ and KIFC1 reduces <t>proliferation</t> in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing <t>BrdU</t> (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant
    Brdu Cell Proliferation Kit 2750, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brdu+cell+proliferation+assay+kit+2750/brdu+cell+proliferation+assay+kit/pmc12125945-58-0-7
    Average 90 stars, based on 1 article reviews
    brdu cell proliferation kit 2750 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "C/EBPβ increases tumor aggressiveness by enhancing KIFC1 expression in androgen receptor negative triple negative breast cancer"

    Article Title: C/EBPβ increases tumor aggressiveness by enhancing KIFC1 expression in androgen receptor negative triple negative breast cancer

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-025-02243-7

    Silencing of C/EBPβ and KIFC1 reduces proliferation in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing BrdU (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant
    Figure Legend Snippet: Silencing of C/EBPβ and KIFC1 reduces proliferation in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing BrdU (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant

    Techniques Used: Immunofluorescence, Transfection, BrdU Incorporation Assay, Two Tailed Test

    Related Articles

    BrdU Cell Proliferation Assay:

    Article Title: Mechanism of paracrine communications between hepatic progenitor cells and endothelial cells.
    Article Snippet: .. The BrdU Cell Proliferation Assay Kit (2750, Millipore, MA, USA) and the TUNEL Assay Kit - FITC (ab66108, Abcam, Cambridge, UK) were used per the manufacturer’s manual. .. The In Vitro Angiogenesis Assay Kit (ECM625, Millipore, MA, USA) was used to determine the ability of HUVECs to form tubular structures according to the manufacturer’s protocol.

    Article Title: Rational design and synthesis of lumican stapled peptides for promoting corneal wound healing.
    Article Snippet: Purpose: Lumican is a major extracellular matrix (ECM) component in the cornea that is upregulated after injury and promotes corneal wound healing.. We have recently shown that peptides designed based on the 13 C-terminal amino acids of lumican (LumC13 and LumC13C-A) are able to recapitulate the effects of lumican on promoting corneal wound healing.. Herein we used computational chemistry to develop peptide mimetics derived from LumC13C-A with increased stability and halflife that are biologically active and non-toxic, thereby promoting corneal wound healing with increased pharmacological potential.

    Article Title: Isorhamnetin exerts anti-proliferative effect on cancer-associated fibroblasts by inducing cell cycle arrest.
    Article Snippet: Isorhamnetin (ISO), a dietary flavonoid, has been shown to possess antioxidant, anti-cancer, and antiinflammatory properties.. Cancer-associated fibroblasts (CAFs), found in the tumor microenvironment of several types of cancer including pancreatic ductal adenocarcinoma (PDAC) impact the tumor growth and development of chemoresistance.. Thus, modulating CAFs is an attractive mean to increase the efficacy of therapies targeting cancer cells.

    TUNEL Assay:

    Article Title: Mechanism of paracrine communications between hepatic progenitor cells and endothelial cells.
    Article Snippet: .. The BrdU Cell Proliferation Assay Kit (2750, Millipore, MA, USA) and the TUNEL Assay Kit - FITC (ab66108, Abcam, Cambridge, UK) were used per the manufacturer’s manual. .. The In Vitro Angiogenesis Assay Kit (ECM625, Millipore, MA, USA) was used to determine the ability of HUVECs to form tubular structures according to the manufacturer’s protocol.



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    Silencing of C/EBPβ and KIFC1 reduces <t>proliferation</t> in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing <t>BrdU</t> (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant
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    Image Search Results


    Silencing of C/EBPβ and KIFC1 reduces proliferation in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing BrdU (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant

    Journal: Cell Communication and Signaling : CCS

    Article Title: C/EBPβ increases tumor aggressiveness by enhancing KIFC1 expression in androgen receptor negative triple negative breast cancer

    doi: 10.1186/s12964-025-02243-7

    Figure Lengend Snippet: Silencing of C/EBPβ and KIFC1 reduces proliferation in AR-TNBC cells. ( A – G ) Representative immunofluorescence images ( A – D ) and quantification bar graphs ( E – G ) showing BrdU (green) incorporation in AR + TNBC ( A , B ) and AR-TNBC ( C , D ) cells transfected with scrambled or C/EBPβ siRNA ( A – D , E ) or treated with CW069 ( A – D , G ). Nuclei were counterstained with Hoechst (blue) and α-tubulin (red). ( H – J ) Bar graphs showing BrdU incorporation in cells transfected with scrambled siRNA, C/EBP siRNA ( H ), KIFC1 siRNA ( I ), or treated with CW069 ( J ). Absorbance was measured at 450–540 nm. A : HCC70, B : MFM223, C : HCC1806, D : BT20; A , B -AR-positive TNBC, C , D-AR-negative TNBC. Bars indicate mean ± SEM. Unpaired two-tailed Student’s t -test with Welch’s correction was used to determine statistical significance. * P < 0.05, ** P < 0.005, ns = non-significant

    Article Snippet: BrdU cell proliferation kit , 2750 , EMD Millipore.

    Techniques: Immunofluorescence, Transfection, BrdU Incorporation Assay, Two Tailed Test

    Representative immunofluorescence images ( A ) and bar graphs ( B ) showing BrdU staining in control and KIFC1 KO AA and EA TNBC cell lines. ( C , D ) Representative images ( C ) and bar graphs ( D ) showing invading control and KIFC1 KO AA and EA TNBC cells stained with crystal violet. Representative images ( E ) and bar graphs ( F ) showing wound closure in control and KIFC1 KO AA and EA TNBC cells. ns-non-significant, **- p < 0.005

    Journal: Cell Communication and Signaling : CCS

    Article Title: A novel role for KIFC1-MYH9 interaction in triple-negative breast cancer aggressiveness and racial disparity

    doi: 10.1186/s12964-024-01664-0

    Figure Lengend Snippet: Representative immunofluorescence images ( A ) and bar graphs ( B ) showing BrdU staining in control and KIFC1 KO AA and EA TNBC cell lines. ( C , D ) Representative images ( C ) and bar graphs ( D ) showing invading control and KIFC1 KO AA and EA TNBC cells stained with crystal violet. Representative images ( E ) and bar graphs ( F ) showing wound closure in control and KIFC1 KO AA and EA TNBC cells. ns-non-significant, **- p < 0.005

    Article Snippet: A BrdU cell proliferation kit (EMD Millipore, #2750) was used to assess cell proliferation.

    Techniques: Immunofluorescence, BrdU Staining, Control, Staining